Abstract: In recent years, Jiaosu products on the market mainly include liquid, paste, powder, tablet and other product forms. Among them, Jiaosu powder with probiotics has become the promising variety of Jiaosu serial products due to its feature of easy preservation and friendly transportation. However, there are some issues with the existing product such as low density and activity, these issues restrict its development. Thus, Jiaosu powder containing probiotics with high density and activity has become one of the notable trends. In order to improve the viability of probiotics in Jiaosu powder and the activity of the product, preparation and optimization of high activity Jiaosu powder with probiotics and the bio-activity of the products was investigated in this study. Lactobacillus plantarum 1-33 (Lp 1-33) was used as the microbial starter culture. Firstly, high density cultivation was used for bacteria enrichment. and the fermentation broth was centrifuged to obtain the cell free supernatant and bacteria pellets. According to the different requirements of the drying effects of the supernatant and bacteria, this study combined two drying technique to reduce the cost and and maximize the protection effects. The cell free supernatant was treated by spray drying. The bacteria pellets were microencapsulated by endogenous emulsification with optimized calcium carriers and appropriate sodium alginate concentration and coating with chitosan to provide extra protection for the strain. Then the microcapsules were treated by vacuum freeze drying. Finally, the two products were mixed and pelleted as granules to form the Jiaosu powder. The microencapsulation efficiency (ME), gastrointestinal survival rate and release rate were used as indicators to investigate the protective effect of multiple protection techniques on biological activity. The results showed the endogenous emulsification was an effective method for microcapsulation using EDTA-Ca as the calcium carrier, 1.5% sodium alginate as the wall material, and 0.3% chitosan-sodium tripolyphosphate as the coating material. Under these conditions, the microencapsulation efficiency of prepared microcapsule was more than 80%; After treatment with artificial simulated gastric juice (SGJ) and simulated intestinal juice (SIJ) for 2 h, the survival rate of Lactobacillus plantarum 1-33 was more than 50%, and the release rate was greater than 90%. These results indicated large amounts of viable cells of Lactobacillus plantarum were obtained after acid stress of gastric juice and could be directional released in the intestinal tract. According to single factor and orthogonal experiment, the best formula of cryoprotectants was: 10% skim milk powder, 8% lactose, 1% sodium ascorbate, 1% sodium glutamate and the ratio of bacterial powder to cryoprotectant is 1:10. Then the prepared probiotics Jiaosu powder was stored at 30 ℃ for 3 months, and the amount of Lactobacillus plantarum 1-33 is still more than 9.5 lg(CFU/g); Compared with the pre-fermentation juice, free-radical scavenging activity of probiotics Jiaosu powder increased by nearly 20%. Through the above multiple active protection techniques, the survival rate and stress tolerance of bacteria are improved, and a new kind of probiotics Jiaosu powder with high density and high activity is obtained, which provides technical support for the subsequent industrial production.