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杨希娟,党斌,张杰,张文刚,陈丹硕.黑青稞麸皮结合态酚类物质大孔树脂分离纯化工艺优化[J].农业工程学报,2018,34(21):295-303.DOI:10.11975/j.issn.1002-6819.2018.21.037
黑青稞麸皮结合态酚类物质大孔树脂分离纯化工艺优化
投稿时间:2018-07-08  修订日期:2018-09-14
中文关键词:  粮食    树脂  黑青稞  结合酚  大孔树脂  分离纯化  抗氧化活性
基金项目:青海省科技厅应用基础项目(2016-ZJ-711);北京大学翁洪武科研原创基金(2018年度);青海省重点实验室科技创新平台建设项目(1-6);青海省“高端创新人才千人计划”(2016年度);青海省第二批 “135”高层次人才培养计划(2017年度)
作者单位
杨希娟 1. 青海大学农林科学院西宁 8100162. 青海省青藏高原农产品加工重点实验室西宁 8100163. 青海大学省部共建三江源生态与高原农牧业国家重点实验室西宁 810016 
党斌 1. 青海大学农林科学院西宁 8100162. 青海省青藏高原农产品加工重点实验室西宁 8100163. 青海大学省部共建三江源生态与高原农牧业国家重点实验室西宁 810016 
张杰 1. 青海大学农林科学院西宁 8100162. 青海省青藏高原农产品加工重点实验室西宁 810016 
张文刚 1. 青海大学农林科学院西宁 8100162. 青海省青藏高原农产品加工重点实验室西宁 810016 
陈丹硕 4. 青海省青稞资源综合利用工程技术研究中心西宁 810016 
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中文摘要:为了筛选对黑青稞麸皮结合酚类物质具有良好吸附、解吸性能的树脂,并建立其分离纯化工艺,评价其体外抗氧化活性,提高黑青稞麸皮的加工利用价值。本研究通过静态吸附和解吸试验比较了10种大孔树脂对黑青稞麸皮结合酚中总酚和总黄酮的分离纯化效果,筛选出AB-8为最佳吸附树脂类型,其静态吸附4 h可达到饱和;优化的吸附和解吸工艺参数为:黑青稞麸皮结合酚提取液pH值为3.0,上样质量浓度1.5 mg/mL,上样速度为1.5 mL/min,60%乙醇溶液作为洗脱剂进行动态洗脱,洗脱流速为1.5 mL/min;优化工艺条件下,经LC/MS检测,AB-8大孔树脂能显著提高71.43%以上不同种类单体酚的含量,且阿魏酸、丁香酸、苯甲酸、鞣花酸、杨梅素和芦丁是纯化后黑青稞麸皮结合酚中的主要酚类物质;体外抗氧化活性表明,黑青稞麸皮结合酚粗提物和纯化物均具有较强的体外抗氧化活性,纯化后的黑青稞麸皮结合酚溶液清除DPPH·、ABTS·+自由基及FRAP铁离子还原能力均显著增强。研究结果表明AB-8大孔树脂对黑青稞麸皮结合酚中总酚和总黄酮有较好的分离纯化效果,具有潜在的工业应用前景。
Yang Xijuan,Dang Bin,Zhang Jie,Zhang Wengang,Chen Danshuo.Process optimization on separation and purification of bound polyphenol in black highland barley bran by macroporous resin[J].Transactions of the Chinese Society of Agricultural Engineering (Transactions of the CSAE),2018,34(21):295-303.DOI:10.11975/j.issn.1002-6819.2018.21.037
Process optimization on separation and purification of bound polyphenol in black highland barley bran by macroporous resin
Author NameAffiliation
Yang Xijuan 1. Academy of Agricultural and Forestry Sciences, Qinghai University, Xining 810016, China
2. Tibetan Plateau Key Laboratory of Agric-Product Processing, Xining 810016, China
3. State Key Laboratory of Plateau Ecology and Agriculture, Qinghai University, Xining 810016, China
 
Dang Bin 1. Academy of Agricultural and Forestry Sciences, Qinghai University, Xining 810016, China
2. Tibetan Plateau Key Laboratory of Agric-Product Processing, Xining 810016, China
3. State Key Laboratory of Plateau Ecology and Agriculture, Qinghai University, Xining 810016, China
 
Zhang Jie 1. Academy of Agricultural and Forestry Sciences, Qinghai University, Xining 810016, China
2. Tibetan Plateau Key Laboratory of Agric-Product Processing, Xining 810016, China
 
Zhang Wengang 1. Academy of Agricultural and Forestry Sciences, Qinghai University, Xining 810016, China
2. Tibetan Plateau Key Laboratory of Agric-Product Processing, Xining 810016, China
 
Chen Danshuo 4. Qinghai Engineering Technology Research Center of Highland Barley Resources Comprehensive Utilization, Xining 810016, China 
Key words:grain  polyphenols  resins  black highland barley  bound polyphenol  macroporous resin  separation and purification  antioxidant activity
Abstract: Highland barley grows in the Qinghai-Tibet Plateau at an elevation of 1 400~4 700 m, and it is the major crop in the Qinghai-Tibet Plateau region, as well as the main food for farmers and herdsmen in Tibetan areas for sustenance. Black highland barley is a precious germplasm resource. Black highland barley has attracted extensive attention due to its rich nutrition and functional chemical components in the Qinghai-Tibet Plateau region. It has been developed into various types of food. But in the process of black highland barley, lots of brans were produced and wasted. The black barley bran is rich in phenolic compounds including phenolic acids and flavonoids. At present, most of the reports on polyphenols in black highland barley have been about free phenolics. There are few reports about bound phenolics. There have been no reports regarding research on separation and purification of bound phenolic compounds and their antioxidant activities in black highland barley bran from the Qinghai-Tibet Plateau region. This paper focuses on the separation and purification technology of bound polyphenols in barley highland bran and its antioxidant activity. The aim of this study was selecting a resin which has a good adsorption and desorption performance of phenols of black highland barley bran, optimizing the separation process parameters and evaluating its antioxidant activity in vitro, thus improving value of processing and utilization about black highland barley barley bran. The separation and purification process of black highland barley barley bran polyphenol was established by macroporous resin. The static adsorption and desorption performance of ten different polarities macroporous resins (NKA-9, NKA-2, HPD-826, HPD-100, H1020, D101, X-5 and AB-8) to total phenolics and flavonoids of black highland barley barley bran bound extraction were compared to select suitable resin for purification of phenolic compounds. AB-8 macroporous resin exhibited the best capability of adsorption and desorption of total phenolics and total flavonoids in black highland barley bran, it was selected as the best adsorption resin type of separation and purification of bound phenolics. And the macroporous resin reached equilibrium within 4 h. The optimum conditions for adsorption and desorption of bound phenolics were as following: pH value of black highland barley bran bound phenolic extract was adjustment to 3.0, sample concentration was 1.5 mg/mL, the polyphenol was passed through the AB-8 resin at a flow rate of 1.5 mL/min, followed by desorption with 60% ethanol at a flow rate of 1.5 mL/min. Under optimum technology conditions, Ab-8 macroporous resin could significantly increase the content of over 71.43% individual phenolics, the most abundant bound phenolics were ferulic acid, syringic acid, benzoic acid, ellagic acid, myricetin and rutin in the purified black highland barley bran. Among the three antioxidant systems, the crude and purified extracts of bound polyphenols in black highland barley bran both showed a relatively higher capacity to scavenge DPPH( and ABTS(+. It also had a relatively stronger ferric reducing antioxidant power (FRAP). But the antioxidant capacity of the purified bound polyphenol performed was better than crude sample on this study. In conclusion, AB-8 macroporous resin could be applied to purify total phenolics and total flavonoids of bound polyphenol in litchi pulp black highland barley bran. It has potential industrial application prospect.
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