Abstract: Abstract: Lentinusedodes stipe contains a lot of dietary fiber and has the double function as the food and herb medicine. Aiming at the current problems such as low efficiency, poor quality, poorly suited to industrial production etc. during the flossing process of lentinusedodes stipe. In this paper, we developed a floss machine of lentinusedodes stipe based on the high-speed impact and floss mechanism. The machine broke the samples to small floss based on the collision and rubs between the high-speed rotating blades and lentinusedodes stipe, and made lentinusedodes stipe to the floss. The floss machine of lentinusedodes stipe mainly included rack, feed hopper device, floss cylinder, the main drive device, the floss device, and discharge device. The floss device of lentinusedodes stipe located in the flossing cylinder was mainly composed of the spindle, the blade wheels, the sleeves and the cutters. The blade wheels were installed on the spindle and separated by sleeves, the distance between each adjacent blade wheels was50 mm. Each blade wheel had 4 cutters. The floss cylinder consisted of the upper cylinder and the lower cylinder. The lower cylinder was welded to frame and the upper cylinder was a movable part, the two cylinders were connected with bolts. The floss cylinder was sealed at both ends and the upper and lower cylinder could be separated. The feed hopper device was installed on the upper cylinder and the lower cylinder had a discharge hole. The discharge device was mainly composed of fan, motor, discharge hole and centrifugal dust collector. The fan was connected to the spindle of the motor, and the air intake of the fan was connected to the discharge hole by air pipe, and the centrifugal dust collector connected discharge hole of the fan. The main drive device was composed of motor, big belt wheel, small belt wheel and V-belt. The big belt wheel was installed on the spindle of the motor, and the transmission ratio of the main drive device was 2:1. The machine could achieve continuous and industrial production of lentinusedodes stipe. In order to study effects of the different process and structure parameters on the performance of the floss machine, we chose types of the fresh and dried lentinusedodes stipe, moisture content of lentinusedodesstipe, the spindle speed, types of cutters, the distance between the end of the cutters and the floss cylinder wall as the experimental factors, and the floss content, fill power and sensory evaluation as the evaluation indexes, to conduct the floss tests of lentinusedodes stipe. The test results showed that for the floss content, the moisture content of lentinusedodes stipe, the spindle speed and the types of the fresh and dried lentinusedodes stipe had a highly significant effect (P<0.01). Other factors had a significant effect(P<0.05). For the fill power, the moisture content of lentinusedodes stipe, the types of the fresh and dried lentinusedodes stipe and the distance between the end of the cutters, and the wall of the floss cylinder had a highly significant effect(P<0.01), but the types of cutters, and the spindle speed had a significant effect (P<0.05). For the sensory evaluation, the moisture content oflentinusedodesstipe, the types of cutters, the spindle speed and the distance between the end of the cutters and the wall of the floss cylinder had a highly significant effect (P<0.01), but the types of the fresh and dried lentinusedodes stipe had an insignificant effect (P>0.05). Based on the above analysis, we conducted orthogonal experiment and comprehensively analyzed the floss content, fill power and sensory evaluation based on the comprehensive evaluation method, and determined the optimal level combination of the floss tests. The results showed that the dried lentinusedodes stipe, the moisture content of dried lentinusedodes stipe was about 50%, spindle speed was about 2 200 r/min, the type of cutter was flat cutter, the distance between the end of the cutters and floss cylinder wall was7 mm. The best test result was the average floss content was 97.1%, the average fill power was 0.079 g/cm3, and the average sensory evaluation score was 16.8.