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苏东林,单 杨,李高阳,张菊华,张 群.酶法辅助提取柑桔皮总黄酮的工艺优化研究[J].农业工程学报,2008,24(4):.DOI:
酶法辅助提取柑桔皮总黄酮的工艺优化研究
投稿时间:2007-05-24  修订日期:2008-02-19
中文关键词:  柑桔皮  总黄酮  纤维素酶  数学模型  响应面法
基金项目:国家高技术研究发展计划(2007AA10Z307);湖南省“十一五”重大科技专项(2006NK1002);湖南省农业科学院“青年学术带头人培养计划”(20051510)
作者单位
苏东林 湖南省农产品加工研究所长沙 410125 国家柑橘加工技术专业研发分中心长沙 410125国家柑橘质量安全监督检验中心长沙 410125 
单 杨 湖南省农产品加工研究所长沙 410125 国家柑橘加工技术专业研发分中心长沙 410125国家柑橘质量安全监督检验中心长沙 410126 
李高阳 湖南省农产品加工研究所长沙 410125 国家柑橘加工技术专业研发分中心长沙 410125国家柑橘质量安全监督检验中心长沙 410127 
张菊华 湖南省农产品加工研究所长沙 410125 国家柑橘加工技术专业研发分中心长沙 410125国家柑橘质量安全监督检验中心长沙 410128 
张 群 湖南省农产品加工研究所长沙 410125 国家柑橘加工技术专业研发分中心长沙 410125国家柑橘质量安全监督检验中心长沙 410129 
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中文摘要:为了充分利用农业废弃物柑桔皮,进一步提高柑桔皮中总黄酮的提取效率,在单因素试验的基础上,采用中心组合设计对柑桔皮总黄酮纤维素酶法辅助提取工艺中的提取时间、提取温度、pH值和酶用量4因子的最优化组合进行了定量研究,建立并分析了各因子与黄酮得率关系的数学模型。结果表明:最佳的工艺条件为酶用量0.55%、提取时间123 min、pH值4.4、温度48℃。经试验验证,在此条件下,黄酮得率为(3.51±0.13)%,与理论计算值3.62%基本一致。说明回归模型能较好地预测柑桔皮中总黄酮的提取得率。
Su Donglin,Shan Yang,Li Gaoyang,Zhang Juhua,Zhang Qun.Technology optimization for extracting total flavonoids from citrus peel by enzyme-assisted technique[J].Transactions of the Chinese Society of Agricultural Engineering (Transactions of the CSAE),2008,24(4):.DOI:
Technology optimization for extracting total flavonoids from citrus peel by enzyme-assisted technique
Author NameAffiliation
Su Donglin 1. Hunan Agricultural Product Processing Institute, Changsha 410125, China
2. National Citrus Processing Technology Research and Development Center, Changsha 410125, China
3. National Citrus Quality Safety Supervision and Test Center, Changsha 410125, China 
Shan Yang 1. Hunan Agricultural Product Processing Institute, Changsha 410125, China
2. National Citrus Processing Technology Research and Development Center, Changsha 410125, China
3. National Citrus Quality Safety Supervision and Test Center, Changsha 410125, China 
Li Gaoyang 1. Hunan Agricultural Product Processing Institute, Changsha 410125, China
2. National Citrus Processing Technology Research and Development Center, Changsha 410125, China
3. National Citrus Quality Safety Supervision and Test Center, Changsha 410125, China 
Zhang Juhua 1. Hunan Agricultural Product Processing Institute, Changsha 410125, China
2. National Citrus Processing Technology Research and Development Center, Changsha 410125, China
3. National Citrus Quality Safety Supervision and Test Center, Changsha 410125, China 
Zhang Qun 1. Hunan Agricultural Product Processing Institute, Changsha 410125, China
2. National Citrus Processing Technology Research and Development Center, Changsha 410125, China
3. National Citrus Quality Safety Supervision and Test Center, Changsha 410125, China 
Key words:citrus peel  total flavonoids  cellulase  mathematical model  response surface methodology
Abstract:In order to make full use of agricultural waste materials-citrus peel and increase the extraction efficiency of total flavonoids from citrus peel, the influences of extraction time, extraction temperature, pH value and the mass ratio of cellulase to material on the yield of total flavonoids from citrus peel with the enzyme-assisted extraction technique were studied according to the central composite experimental design based on the single factor experiments. One mathematical model established and analyzed by response surface methodology(RSM) was adequate to describe the relationships between the studied factors and the response of total flavonoids extraction yield. Based on the canonical analysis, the optimum extraction conditions were obtained as follows: the added ratio of cellulose 0.55%, the extraction time 123 minutes, pH value 4.4 and the temperature 48℃. Under the optimized extraction conditions, it can be included that the flavonoids yield is (3.51±0.13)% (n=3), which is close to the estimated value 3.62% attained by using regression model. This model can be used to predict the experiment results well.
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