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彭源德,郑科,杨喜爱,严理,唐守伟,朱爱国,熊和平.苎麻纤维质酶降解生产生物燃料乙醇的工艺[J].农业工程学报,2007,23(4):6-10.DOI:
苎麻纤维质酶降解生产生物燃料乙醇的工艺
投稿时间:2006-06-13  修订日期:2007-03-20
中文关键词:  苎麻  纤维质  酶降解  影响因素
基金项目:国家948项目(2006-G18);中国农业科学院科技经费课题和湖南省麻类作物遗传育种与麻产品生物加工重点实验室课题“苎麻生产燃料酒精的酶糖化技术基础研究”的部分研究内容
作者单位
彭源德 (1965-)研究员主要从事农产品生物加工研究。长沙中国农业科学院麻类研究所410205。Email:ibcpyd313@126.com 
郑科 中国农业科学院麻类研究所长沙 410205 
杨喜爱 中国农业科学院麻类研究所长沙 410205 
严理 中国农业科学院麻类研究所长沙 410205 
唐守伟 中国农业科学院麻类研究所长沙 410205 
朱爱国 中国农业科学院麻类研究所长沙 410205 
熊和平 研究员博士生导师研究方向为麻类酶加工研究。长沙中国农业科学院麻类研究所410205。Email:Ramiexhp@2118.cn 
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中文摘要:在微生物预处理苎麻韧皮的基础上,进行了pH值、酶用量、葡萄糖浓度和原料处理方式等对苎麻纤维质酶降解的单因子试验和正交试验。结果表明,苎麻纤维质酶解的起始pH值为5.0左右,总糖转化率最高,达72.361%;在一定的酶浓度范围内,随着酶量的增加,苎麻纤维质的总糖转化率提高,酶的适宜用量为:木聚糖酶5%、纤维素酶10%;水解液中葡萄糖浓度在0.5%以下时,苎麻纤维质的总糖转化率不受影响,葡萄糖浓度在0.5%以上时,随着水解液中葡萄糖含量的增加,总糖转化率下降;苎麻纤维质酶解过程中,对原料进行机械处理,有利于提高酶的水解效率;苎麻纤维质酶解的最适条件为:pH值4.5~5.5、纤维素酶浓度8%~11%、葡萄糖浓度小于0.5%、原料不洗直接进行剪碎处理。
Peng Yuande,Zheng Ke,Yang Xi′ai,Yan Li,Tang Shouwei,Zhu Aiguo,Xiong Heping.Technology for producing bio-ethanol from ramie lignoellulosic degradation with enzymes[J].Transactions of the Chinese Society of Agricultural Engineering (Transactions of the CSAE),2007,23(4):6-10.DOI:
Technology for producing bio-ethanol from ramie lignoellulosic degradation with enzymes
Author NameAffiliation
Peng Yuande Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Changsha, Hunan 410205, China 
Zheng Ke Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Changsha, Hunan 410205, China 
Yang Xi′ai Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Changsha, Hunan 410205, China 
Yan Li Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Changsha, Hunan 410205, China 
Tang Shouwei Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Changsha, Hunan 410205, China 
Zhu Aiguo Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Changsha, Hunan 410205, China 
Xiong Heping Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Changsha, Hunan 410205, China 
Key words:ramie  lignocellulose  enzymatic degrading  influential factors
Abstract:After being pretreated on ramie bast fiber by microbial retting, both single factor and multi-factors were experimented during ramie lignocellulosic degrading with enzymes, these influential factors are pH value, concentrations of enzymes and glucose, and methods for material treatment. The results show that, the highest rate of saccharification reaches 72.361% at the beginning of pH 5.0; Within a range of enzymes concentration, the rate of saccharification can be increased with the increase of enzymes; The optimal enzyme concentrations are 5% of xylanase and 10% of cellulase, respectively; The rate of saccharification is not influenced as glucose concentration in the solution of lignocellulosic degrading is below 0.5%, and as over 0.5% of glucose concentration and with the increase of glucose in the solution, the rate of saccharification is decreased; Mechanical treatment on material is beneficial for improving the efficiency of enzymatic degrading. The most optimum conditions for ramie enzymatic lignocellulosic degrading is pH 4.5~5.5, 8%~11% of cellulose concentration, below 0.5% of glucose concentration and treatment of scissorssed and unwatered material.
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