Abstract: Environment monitoring of cold-chain logistics for fruits and vegetables has the requirement of multi-point, multi-factor and convenience. The JN5139 based on Zigbee was selected as the core of information processing of wireless node, and the sensing node was designed combined with the temperature and humidity sensors. Then a wireless sensor network in the vehicle environment was constructed. Furthermore, the environmental information collecting software was designed with the embedded system and .net platform, which realized data collection and real-time monitoring. The tests of PRR (packet reception rate) were carried out under different positions of cold chain vehicle, with different temperatures (0, 5 and 12℃) at 90% relative humidity. The results showed that working performance of the system was stable and the functions of real-time data acquisition and transmission could meet the design requirements. The system can be easily applied in environmental information collection and monitoring during the cold-chain transportion.