Abstract: To confirm gain effect of heating temperature on phototactic response of locusts, and coupling effect of light and heating temperature inducing locusts effectively, influence of the same illumination of violet light and green–violet–blue light combined with different heating temperatures were investigated through the comparative test of locust’s directional response to LED light and coupling of light and temperature by means of LED light source, heating device and locust’s phototactic trial equipment. The coupling effect of light and heating temperature interacting with environmental temperature influenced on the principle of the locust’s phototactic gain was dicussed. Heating temperature, locust’s sensitive spectral illumination, environmental temperature, activive behavioral rhythm of locusts, behaving during the daytime and habitating at night have influences on locust’s phototactic selective response. The heating temperature of 65℃ combined with different spectrum has better effect on locust’s phototactic gain. Simultaneously, locust’s phototactic selective response to the coupling effect of violet light and heating temperature of 65℃, comparing coupling effect of different spectral light and different heating temperature, is the best during the same time periods, and the gain effect of heating temperature on locust’s phototactic response at 0:00-2:00 is the optimum. Therefore, on phototactic response of locusts inspired by coupling effect of locust’s sensitive spectral illumination and heating temperature, heating temperature on locust’s phototactic response and cluster has the function of, gain effect while locust’s sensitive spectral illumination plays a major role. The results can supply a theoretical foundation for design of photoelectric stimulation sources used in photoelectric inducing and trapping locusts.