玉米单倍体核磁共振自动分拣系统的开发

Development of automatic nuclear magnetic resonance screening system for haploid kernels in maize

摘要: 摘要：为满足玉米单倍体育种与研究的大规模应用，本研究利用玉米油份花粉直感效应的生物学特性，开发了玉米孤雌生殖诱导单倍体核磁共振自动识别分拣系统。该系统由单籽粒自动进样模块、玉米油份自动测试模块和自动分拣模块三大模块组成。结果证实该玉米单倍体核磁共振自动分拣系统具有快速（平均速度为4秒/粒）、准确（平均为92.3%）、自动、智能分拣玉米单倍体的优良特性，同时，本系统也可应用于基于油份差异的其他样品的自动智能化筛选。

Abstract: An automatic screening system for maize haploid kernel identification based on oil xenia effect and nuclear magnetic resonance (NMR) is developed to meet the demand of large-scale application in maize haploid breeding and research. The screening system was comprised of a seed feeding module, a measurement module of oil content and a screening module. The results show that the mean accuracy of this screening system reaches 92.3%. Even with the shorter signal acquisition time, the accuracy does not show a significant decline. The automatic screening system can be used in practical application in rapid selection of maize haploid seeds and can also be used to separate the samples of different oil contents.