Abstract: Hyperspectral imaging (HSI) technology has been used widely in agriculture and food industry. The paper discussed the integration and calibration methods for a line-scan HSI system, such as the sequence of the system adjustment, scanning speed and position control of the linear slide, spectral accuracy calibration, and spatial distortion calibration of the spectral image. Detailed methods and steps were provided to ensure the high fidelity of the image. The "smile" and "keystone" distortions of the spectral images were also taken into account. The calibration results showed that the spectral and spatial errors were 1.26 nm and 0.03 mm, respectively. A quadratic equation was used to correct the wavelength positions based on the standard spectrum of a Krypton lamp. It is proved that this method provided preferences to prepare image acquisition system quickly and efficiently.