Abstract: In order to study the fond habit of juvenile turbot to different color backgrounds, the experiment of 9 color backgrounds selection was conducted and fish body color response to different colors was analyzed. The results showed that the appearance frequency of juvenile turbot in purple and pink backgrounds was significantly higher than that in black and red ones, and body brightness change rate under black and red backgrounds was more drastic and intensive than under pink and purple backgrounds. Results indicated that fish needed more physiological response to adapt black or red background and it is better to use lighter color as the background rather than black or red color. This research provides reference for aquaculture system designs and operations.