Abstract: In order to help the farmers to acquire the agricultural knowledge by telephones, this paper presents an agricultural knowledge acquisition method based on the coupling of Call center and expert system. This method adopts the implementation plan of call center database trigger technology and expert system event monitor technology, achieves the call center IVR flow to transfer the expert system knowledge base automatically, so as to reduce the massive system development workload by not redesigning diagnosis algorithm and knowledge base for call center. This method also coordinates the call center CTI server and traditional database server and web server, to help farmers to acquire agricultural knowledge their need with the aid of the telephone network.