高压脉冲电场对乳铁蛋白铁结合能力的影响(简报)

卢蓉蓉; 陈梅仙; 杨瑞金; 张文斌

高压脉冲电场对乳铁蛋白铁结合能力的影响(简报)

Effects of high-intensity pulsed electric fields on the iron-binding capacity of lactoferrin

摘要

摘要: 为了探讨高压脉冲电场（PEF）处理对生物活性蛋白功能性质的影响，为这种新型的冷杀菌技术的实践应用提供理论指导，该文选取乳铁蛋白（LF）为研究对象，采用分光光度法测定其铁结合能力，分析了PEF处理中各处理参数对乳铁蛋白铁结合性能的影响。研究结果表明，在该试验设定条件下，LF的铁结合能力大体随电场强度、处理时间和脉冲宽度增加而降低。电场强度为35 kV/cm时，LF的铁结合能力降低至对照样的57%左右。随处理时间的增加，LF铁结合能力下降。脉冲宽度为4 ms时，LF的铁结合能力下降为48％。当脉冲数为256时，LF的铁结合能力达到对照样的3.8倍。当处理温度上升到55℃时，LF铁结合能力为对照样的1.2倍。可见，PEF处理会对生物活性蛋白的功能性质产生正面或负面的影响，选择适宜的操作参数，有望提高其生物活性功能。

Abstract: To investigate the influence of high-intensity Pulsed Electric Fields (PEF) on the functional property of bioactive protein, and to provide theoretical basis for application of this new cold sterilization technology, the effect of PEF on the iron-binding capacity of Lactoferrin (LF) was investigated. The iron-binding capacity of LF was determined by spectrophotometry method. The results indicated that the iron-binding capacity generally decreased with the increase of electric field strength, treatment time and pulse width. The iron-binding capacity decreased by 57% or 48% after PEF treatment at 35 kV/cm or 4 μs, and decreased with the increase of treatment time. Whereas it increased by 3.8 times or 1.2 times at 256 pulse amount or 55℃, respectively. It could be concluded that PEF treatment had positive or negative effect on the functional properties of bioactive protein. It indicates that the functional property of bioactive protein can be increased by reasonable PEF treatment.

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