猕猴桃果实贮藏期特性对蓝莓-猕猴桃复合果浆品质的影响

    Effect of the kiwifruit’s properties during storage on the quality of blueberry-kiwifruit composite pulp

    • 摘要: 为解决蓝莓果浆在加工和贮藏过程中易发生絮凝及稳定性劣化从而制约其产业化应用问题,引入猕猴桃果浆构建天然稳定复合体系,显著改善蓝莓果浆流变特性、提升稳定性。基于猕猴桃果实贮藏期间(10 d)关键品质动态演变规律,将贮藏1~10 d的猕猴桃制浆后分别与蓝莓果浆按1:9比例复合,并深入解析其对蓝莓-猕猴桃复合果浆品质的影响机制。试验结果表明,随着贮藏期的延长,猕猴桃果肉中淀粉颗粒逐渐水解,可溶性固形物(total soluble solids,TSS)呈先升高后降低的趋势,在贮藏中期(第7 天)达到最大值(14.7 %);细胞壁完整性逐渐降低,阻抗和电抗均呈下降趋势,宏观表现为果肉硬度和黏弹性降低。深入分析蓝莓-猕猴桃复合果浆品质发现,随着猕猴桃果实贮藏期的延长,复合果浆的pH值不断升高,电导率先下降后升高。猕猴桃果浆的加入能够改善蓝莓果浆的凝聚分层现象,其中蓝莓-猕猴桃-7 d试验组表现出较低的表观黏度,为4404.3 Pa·s。粒径分析表明,复合果浆的体积平均径(D4,3)和面积平均径(D3,2)较纯蓝莓果浆减小,说明复合果浆中粒子间结合更为紧密。此外,基于猕猴桃果肉中果胶甲酯酶抑制剂(pectin methylesterase inhibitor,PMEI)的作用,热杀菌后复合果浆中果胶甲酯酶(pectin methylesterase,PME)活性相比蓝莓果浆降低了38.14%,这可能是猕猴桃-蓝莓复合果浆良好流变特性形成的原因。综上,贮藏中期的猕猴桃果实更有利于复合果浆流变学特性的提升,该结果为解决蓝莓果浆絮凝及稳定性劣化提供了解决方案。

       

      Abstract: Blueberry pulp can be prone to phase separation, flocculation, and stability deterioration during processing and storage, thus constraining its large-scale industry. It is an urgent need to develop naturally stabilized composites. Preliminary studies have revealed that the introduction of kiwifruit pulp can be expected to significantly modify the rheological properties of blueberry pulp, in order to improve the stability. In this study, a systematic investigation was made of the dynamic evolution patterns of the key quality attributes in kiwifruit during storage. The underlying mechanisms were determined to govern their impact on the quality of blueberry-kiwifruit pulp composites. The quality features of kiwifruit were first explored (including soluble solids, texture, electrical properties, microstructure, and flavor) during storage. And then, an analysis was implemented on the effects of kiwifruit under different storage conditions on the quality of blueberry-kiwifruit composite fruit (including pH, conductivity, rheological properties, particle size distribution, and PME activity). Results showed that the total soluble solids (TSS) of kiwifruit firstly increased and then decreased with the prolongation of the storage, due to the degradation of starch particles. And the highest TSS (14.7 %) was observed in the kiwifruit storage for 7 days. Additionally, the starch granules in kiwifruit pulp were first degraded, followed by the hydrolysis of cell wall polysaccharides (such as pectin), which were accompanied by the disruption of the pectin network structure during storage. Therefore, the two key quality indicators of kiwifruit gradually decreased, namely, hardness and cohesiveness, indicating the gradual softening in the macro-texture of kiwifruit. Specifically, both the W1W and W5S response values significantly increased as the storage of kiwifruit was prolonged. In detail, the highest W1W response value (18.255) and W5S response values (4.234) were observed in kiwifruit after 10-day storage. Furthermore, the W1W and W5S response values decreased by 80.89% and 41.99%, respectively, thus subjecting to mingling blueberry pulp with kiwifruit pulp. The reason was that the dilution of the kiwifruit pulp was used to inhibit the concentration of volatile flavor compounds in blueberry pulp. The integrity of the cell wall decreased gradually, while the impedance and reactance shared the downward trend, indicating the decreased hardness and viscoelasticity of kiwifruit. In the blueberry-kiwifruit compound pulp, the pH increased with the increase in storage time, while the conductivity firstly decreased and then increased. The layering and condensation in the compound pulp were significantly improved after the addition of the kiwifruit pulp. The lowest apparent viscosity (4 404.3 Pa·s) was obtained in the compound pulp with kiwifruit that had been kept in storage for 7 days. Particle distribution illustrated that there was a decrease in the Volume-average particle size(D 4,3) and the Area-weighted average particle size (D 3,2) of the compound pulp, compared with the single blueberry pulp. It implies that the pulp particles were intensely coated with each other and then formed smaller complexes. Additionally, due to the PME inhibitor (PMEI) from kiwifruit, the PME activity of compound pulp decreased by 38.14%, compared with blueberry pulp after thermal sterilization. This process contributed to the better rheological properties of the compound pulp. Taken together, the kiwifruit stored during the mid-storage period should be considered as the optimal material to improve the rheological quality of the compound pulp.

       

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