葛铭佳, 张丽媛, 艾佳音, 吉茹, 刘静珂, 刘梦雯, 何玲. 热激和山梨酸钾处理对猕猴桃果实灰霉病的抑制效应[J]. 农业工程学报, 2020, 36(7): 316-323. DOI: 10.11975/j.issn.1002-6819.2020.07.037
    引用本文: 葛铭佳, 张丽媛, 艾佳音, 吉茹, 刘静珂, 刘梦雯, 何玲. 热激和山梨酸钾处理对猕猴桃果实灰霉病的抑制效应[J]. 农业工程学报, 2020, 36(7): 316-323. DOI: 10.11975/j.issn.1002-6819.2020.07.037
    Ge Mingjia, Zhang Liyuan, Ai Jiayin, Ji Ru, Liu Jingke, Liu Mengwen, He Ling. Inhibitory effects of heat water and potassium sorbate on gray mold in postharvest kiwifruit[J]. Transactions of the Chinese Society of Agricultural Engineering (Transactions of the CSAE), 2020, 36(7): 316-323. DOI: 10.11975/j.issn.1002-6819.2020.07.037
    Citation: Ge Mingjia, Zhang Liyuan, Ai Jiayin, Ji Ru, Liu Jingke, Liu Mengwen, He Ling. Inhibitory effects of heat water and potassium sorbate on gray mold in postharvest kiwifruit[J]. Transactions of the Chinese Society of Agricultural Engineering (Transactions of the CSAE), 2020, 36(7): 316-323. DOI: 10.11975/j.issn.1002-6819.2020.07.037

    热激和山梨酸钾处理对猕猴桃果实灰霉病的抑制效应

    Inhibitory effects of heat water and potassium sorbate on gray mold in postharvest kiwifruit

    • 摘要: 为了探究热激和山梨酸钾对离体灰霉菌(Botrytis cinerea)及猕猴桃果实采后灰霉病的抑制效应,用48 ℃热水(Hot Water Treatment,HT)和5 g/L山梨酸钾溶液(Potassium Sorbate,PS)及二者复合(PS+HT)分别处理离体灰霉菌和接种过灰葡萄孢霉的猕猴桃果实。离体试验以不添加山梨酸钾的马铃薯葡萄糖琼脂培养基(Potato Dextrose Agar,PDA)培养不加热处理的灰霉菌悬液为对照,结果表明与对照相比,PS和PS+HT处理均能显著抑制离体灰霉菌菌丝生长(P<0.05)。损伤接种试验以常温即18 ℃下清水浸泡接种灰霉菌的猕猴桃果实为对照,浸泡后果实分别贮藏在0 ℃和24 ℃,结果表明在2种温度贮藏条件下,上述处理均可以显著诱导提高多酚氧化酶、过氧化物酶、几丁质酶和β-1,3-葡聚糖酶活性(P<0.05),从而抑制果实病斑扩散,显著降低果实发病指数(P<0.05)。其中复合处理抑菌效果最佳,而PS、HT单一处理的果实病斑直径在贮藏后期与对照差异不显著(P>0.05)。此外,为了进一步探究上述处理是否对猕猴桃果实冷藏品质产生影响,将对照及不同处理组果实在0 ℃下贮藏90 d,每10 d测定品质相关指标。结果表明,与对照相比,PS+HT复合处理延缓了可滴定酸和维生素C含量的下降,并且显著降低了果实失重率和腐烂率(P<0.05),提高了猕猴桃果实耐贮性,对延缓猕猴桃果实衰老和减轻采后腐烂衰败具有重要的意义。

       

      Abstract: Abstract: The present study investigated the effects of the heat shock treatment and potassium sorbate solution on the suppression of gray mold (Botrytis cinerea) of 'xuxiang' kiwifruits under postharvest conditions. The treatment in the test included potassium sorbate at 5 g/L(PS), hot water at 48°C (HT) for 8 min, and a combining of HT with PS (HT+PS), while the sterile water was applied in the control (CK) group. The test was first conducted on the inhibition effects of PS and HT on the mycelium growth of B. cinerea. The 10 μL of spore suspension was treated with or without HT before the inoculation on potato dextrose agar medium with or without PS treatment. After each treatment, the spore suspensions were further cultured at 24°C for evaluation. After 4 days of in vitro culture, the PS treatment was found to significantly inhibit the growth of mycelium compared with CK (P<0.05). The optimal result was obtained from the HT+PS treatment, after which the observed colony diameter was only 13% that of the CK. In the injury inoculation test, the fruits that inoculated with B. cinerea spore suspension were treated with HT, PS, HT+PS, or clean water for 8 min. The fruits were then dried, and stored at 0°C for 30 days and 24°C for 7 days. The lesion diameter, disease index and the activity of resistance-related enzymes were measured in the inoculated kiwifruits after different treatments. Under both storage conditions (0°C and 24°C), PS, HT and PS+HT treatments significantly reduced the decay of gray mold in kiwifruits compared with CK (P<0.05). The activities of defense-related enzymes including polyphenol oxidase, peroxidase, chitinase and β-1,3-glucanase were remarkably enhanced in the treatment groups compared with the CK. However, no significant difference in the lesion diameter was detected among the PS, HT, and CK treatments after storage (P>0.05). The optimal result was again obtained from the HT+PS treatment, after which the colony diameters were significantly reduced compard with the CK for the fruits treated at 24°C and 0°C, respectively. The incidence index of the PS+HT treatment at 24°C and 0°C was significantly reduced compard with the CK, respectively. The influence of each treatment on the quality of kiwifruit was further investigated. The fresh fruits were air-dried after different treatments, then stored at 0°C±0.5°C for 90 days. The indicators of the fruit quality were tested every 10 days during storage, and the level of soluble solid content in the fruits was maintained in all treatments. However, the decrease of the titratable acid and ascorbic acid was delayed following all treatments compared with the CK. Similarly, at the end of 90-day storage period, the weight loss and decay were significantly reduced in all treatment groups compared with the CK (P<0.05). The optimal results were again achieved with the HT+PS treatment, after which the rates of weight loss and decay were only 2.11% and 0%, respectively, after 90 days of storage, compared with 4.61% and 3.08%, respectively, for the CK group. These findings indicate an important role for heat water and potassium sorbate treatment in delaying the decay and senescence of postharvest kiwifruit.

       

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