Abstract: Abstract In order to understand the frost resistance of leaves in grapevine and provide sufficient information for grapevine breeding, a simplified low temperature exothermic method was applied to investigate the low temperature exothermic characteristics of leaves in grapevine. The useful information of low temperature exothermic curve was utilized to evaluate the frost resistance of grapevine leaves of fifteen different grape varieties. Six parameters including T1(supercooling point), t1 (maintain time of supercooling point), T2 (freezing point), t2 (time when temperature raised from T1 to T2), t3 (maintain time of freezing point) and S(the area of the blade temperature curve and the air temperature baseline) were extracted form low temperature exothermic curves, to identify the frost resistance of different varieties . Correlation analysis was conducted firstly within the six parameters. There was a significantly positive correlation between T1 and T2, S at 0.01 level. There was a significantly positive correlation between T2 and t3 at 0.05 level and a significantly positive correlation with S at 0.01 level. There was a significantly positive correlation between t2 and t3 at 0.05 level. These results indicated that, possibility existed to simplify these parameters. These simplified parameters could be used to assess the frost resistance of grapevine leaves individually or simultaneously. According to the results of one-way analysis of variance, there was no significant difference within different varieties at the level of T1, t1 and t3, indicating that these three indexes could not be used to distinguish the frost resistance of grapevine leaves. Differences existed within fifteen cultivars according to T2, t2 and S by one-way ANOVA analysis, which indicated that T2, t2 and S could be used to assess the frost resistance of grapevine leaves individually. To integrate the information of T2, t2 and S, membership function of T2, t2 and S were calculated for each cultivar and the mean value of membership function of each cultivar was calculated. The range of mean value of membership function was from 0.096(Muscat Hambourg) to 0.865(Summer Black). The means of membership functions were then used for a cluster analysis. The results shown that, fifteen cultivars could be divided into 3 types according to the frost resistance: Muscat Hambourg and Frontenac, the mean value of membership function was 0.113, own the most frost hardiness；Summer black, the mean value of membership function was 0.865, was the most vulnerable to frost；other cultivars, the mean value of membership function was 0.487, own the moderate frost hardiness. Among fifteen grapevine cultivars, the leaves of Muscat Hambourg and Frontenac own the strongest frost resistance. Our results indicated that, the low-temperature exothermic method was feasible in evaluating the frost resistance of grapevine leaves. The super cooling point was not suitable for comparing frost resistance of grapevine leaves, which was different from the conventional knowledgement.