Abstract: Abstract: In this paper, we studied the influence of storage temperature, temperature fluctuation range and fluctuation frequency on the fluorescence quenching properties of CdTe quantum dots (QDs) during cold chain storage and transportation. The results showed that the storage temperature and time had significant effects on the fluorescence quenching of QDs. The color of QDs changed from red to colorless via orange and green. When the storage temperature was 25 ℃, the QDs turned to colorless after 50 hours, while the color was still green under 4 ℃ and -5 ℃, and it changed from red to orange at -20°C. The higher the temperature was, the faster the color changed. In the cold chain simulation test, the QDs turned to colorless after 20 hours and 18 hours respectively when the temperature fluctuated from 4 ℃ to 25 ℃ and 40 ℃ per 30 min., respectively. In the freezing environment at -20 ℃, the QDs turned to colorless after 26 hours and 23 hours respectively when temperature fluctuated to 25 ℃ and 40 ℃. Obviously, the higher the storage temperature was, the faster the QDs quenched. The greater the fluctuation amplitude was, the quicker the color changed, the faster the HSV value changed. Likewise, the higher the fluctuation frequency was, the quicker the color changed and the faster the QDs quenched. In the process of fluorescence quenching, the storage temperature, fluctuation amplitude and frequency have regular effects on the value of hue, saturation and value of the quantum dots. The value of hue increased with time, and the saturation value and value value were stable for a period of time, and then decreased. The higher the temperature, the greater the amplitude and frequency, the faster the saturation value and the value value changed. Regardless of the temperature fluctuation amplitude or the increasing of the fluctuation frequency, it intended to increase the accumulated value of time and temperature, the greater the accumulated time and temperature value and the faster the quench of the QDs. This was similar to the change in food quality. The batch tests of QDs showed that the quench time of different batches showed obvious discrepancy under the same conditions, the first batch turned to colorless after 59 hours under 4 ℃, while the second batch cost 23 hours. The results illustrated that the HSV value of the second batch changed faster than the first one, but it was constant during the quenching process. The study demonstrated that the QDs can be applied to the cold chain monitor by squeezing the color parameters, thus CdTe QDs have a potential for food cold chain storage and transport monitoring. It is also necessary to optimize the preparation process of CdTe QDs so that the fluorescence quenching properties in the cold chain is more similar to that of food quality. It is expected that the time-temperature indicator based on QDs will be applied to food cold chain transport process to characterize the changes of food quality.