Abstract: Abstract: Horticultural products are easy to be infected by microorganisms which often cause great waste of resources and huge loss of economy，so it is imperative to inhibit the activity of the microorganisms in the storage environment of the harvesting horticultural products. As the representative of the Penicillium fungal infection is a major cause for decayed fruits and vegetables. Funfaltoxins derived from Penicillium will do huge harm to people with strong stability and cancerigenic toxin after infecting vegetables and fruits. In this article, we did some research about how to use nano titanium dioxide (TiO2) to inhibit the activity of Penicillium fungal in simulated refrigeration environment TiO2 is a new type of nano semiconductor material, which is widely concerned due to its special properties. When irradiated by excited light, TiO2 would generate electron-hole pairs, then the pairs could produce ?O2? and free radicals (HO?) with high oxidative activity, which can degrade most organic compounds to non-toxic CO2 and H2O. They also have direct effect on bacteria, leading to cell deaths. However, the photo electron and hole is very easy to be combined, which makes the high redox activity lost. This experiment is carried out from two aspects: heavy metals modifying and voltage applying. It is found that heavy metals can change the distribution of the electrons in the surface of TiO2, this can reduce the electron-hole pair of the composite probability and improve the quantum effect of titanium dioxide. When using TiO2 film electrode photo-catalyst to degrade the organic compounds through applying a small positive voltage between the electrolytic tank poles, it could drive the photo-induced electrons in anode flowing to cathode, thus reduces the chance of combination of electrons and holes, resulting in high concentration of hydroxyl radicals and improving the degradation efficiency of organic pollutants. In this study, we choose the activated carbon fiber (ACF) as carrier, nano TiO2 with heavy metal nano-Ag deposition (Ag-TiO2/ACF) were prepared by ion sputtering. The effect of TiO2-ACF with heavy metal Ag modification on the photoelectric synergism for disinfection of Penicillium was also invested. The results showed that the different sputtering treatment in experiments presented different effects and the underlying sputtering exhibited the best performance. The apparent rate constant of Penicillium disinfection reached the highest at 120 s which increased by 39.4% comparing with the contrast. On the other hand, applied voltage can significantly improve the Penicillium disinfection rate of TiO2-ACF film, but less significant improvement for the Ag-ACF/TiO2 film The optimal value are 75 V, and photoelectric synergism effect in this case is better than the use of photo-catalyst or electro- catalyst separately. It can be found that ion sputtering achieved the well distribution of Ag-TiO2 particles on the surface and inside of ACF film when analyze the characterization of ACF film.