Abstract: Abstract: As the increasing demand for efficient and sustainable production and the global demand for reduced antimicrobial drug consumption in Abstract: As the increasing demand for efficient and sustainable production and the global demand for reduced antimicrobial drug consumption in livestock production, the importance of disinfection has increased significantly. However, potentially toxic, corrosive problems have arisen because of the use of chemicals as disinfecting agents. Slightly acidic electrolyzed water (SAEW) which has recently been developed in livestock production is a new environmental friendly disinfectant. In order to make the precision spraying of this disinfectant and increase the disinfectant usage efficiency for reducing residual and disease risk, in this study, the influence of droplets size and deposition on disinfection effect of slightly acidic electrolyzed water was evaluated. Deposition delivered by different atomizers on three aperture types (0.1, 0.3 and 0.5 mm) of sprayer under three levels pressure (30, 50 and 80 MPa), were first measured at 1 minute using a weighing method. The depositions were then divided into different levels with its correspondence pressure and aperture, and the trend influence of disinfection effectiveness of slightly acidic electrolyzed water (SAEW, pH value 6.15-6.35, ACC 135 mg/L), for inactivating Salmonella enteritidis on the surface of clothes, was evaluated at different levels of deposition. In addition, the effect of droplet size on SAEW sprayed disinfection effect was measured with two sprayers, nebulizer (size 80-90 μm) and ultrasonic nebulizer (size ≤30 μm) at different point-central district (A0) and another two different-distance points in the left and right hand (A1, A2). The results showed that deposition constitutes a dense neighborhood at central district (A0), more segmental in another two different-distance points (A1, A2) under the different pressure and aperture types. The deposition amount at the A0 was more than A1 and A2. In addition, the deposition amount at A1 and A2 first increased and then decreased as the pressure and aperture increased. The coefficient of variation from the different points was observed at the same change trend with A1 and A2. The results showed that the deposition significantly affects the log reduction (P<0.05), and the logarithm of reduction of Salmonella was rapidly increased from (1.22±0.02) to (2.09±0.01)lg(CFU/mL), and then change little with the increased deposition. The maximum logarithm of reduction of Salmonella of 2.09±0.01 was finally obtained by the clothes sprayed with SAEW at 1.49×10-2 deposition, and the deposition of 1.49×10-2 g/cm2 may be the best point of deposition for SAEW sprayed disinfection on the surface of clothes. There was a significant difference in the size of droplet on SAEW spraying disinfection effect (P<0.05). It was concluded that the bactericidal effects of the droplet from the nebulizer (size 80-90 μm) at the central target region, was significant higher than the ultrasonic nebulizer (Size≤30 μm) (P<0.05), but the effects of the nebulizers was significant difference at the different locations (P<0.05), and no significant difference (P>0.05) in the bactericidal effects of the ultrasonic nebulizer was observed among different locations. It means the smaller droplet size (size ≤30 μm) had a better uniformity than the larger size droplet (size 80-90 μm)