秸秆与菇渣堆肥用于双孢蘑菇栽培的理化性状和细菌群落分析

    Physical-chemical properties and bacterial community analysis of different composts made by straws or oyster mushroom residues for commerical mushroom Agaricus bisporus

    • 摘要: 该研究以双孢蘑菇(Agaricus bisporus)传统麦草配方作为对照,分别用玉米秸秆和杏鲍菇菇渣替换部分麦草设计2个新配方,通过理化性状、细菌群落动态及产量分析,评价玉米秸秆或杏鲍菇菇渣用于工厂化双孢菇生产的可行性。分别在建堆、一次发酵结束、二次发酵结束3个时期采集堆肥样品,提取总DNA,以大肠杆菌(Escherichia coli)16S rDNA V3区通用引物,进行PCR‐DGGE 扩增和序列分析,同时分析培养料理化性状,并测定双孢蘑菇产量。研究结果:玉米秸秆配方基质颗粒比较粗大,含氮量较低,二次发酵结束时与麦草配方具有较多的共有优势菌群,两者的细菌多样性指数变化规律也基本一致。菇渣配方基质颗粒比较细碎,含氮量较高,其堆肥3个时期的优势细菌种类、多样性指数变化趋势与麦草配方明显不同。传统麦草配方具有良好的理化性状和稳定的细菌群落,玉米秸秆、杏鲍菇菇渣代替部分麦草的配方仍需进一步优化。

       

      Abstract: Abstract: Using the traditional wheat straw formula as control, two test compost formulas were designed using corn straws or oyster mushroom (Pleurotus eryngii) residues replacing part of the wheat straw, respectively. At the beginning of composting, the end of phase I, and phase II, compost samples were collected and total DNA were further obtained. Bacterial communities of each sample were analysed using Polymerase Chain Reaction-Denaturing gradient gel electrophoresis (PCR-DGGE) method based on 16S rDNA using the universal primers from Escherichia coli. The physical-chemical properties of the samples and the yield of the three formula were also measured and analysed. The results showed that the corn stalks formula shared higher particle size and lower nitrogen content than those of the wheat straw formula. At the end of phase II, the corn stalks formula demonstrated considerably high similarity of dominant bacteria with the wheat straw formula. The two formulas also shared similar bacterial diversity variation trends. The oyster mushroom residues formula shared tiny particle size, high nitrogen content. The dominant bacteria species and bacterial diversity variation were highly different from those of the wheat straw formula.

       

    /

    返回文章
    返回